Influence of myosin antiserum on heart sarcolemmal Ca2+ or Mg2+ ATPase activity
Tuana, B.S.; Kutryk, M.J.; Elimban, V.; Wikman-Coffelt, J.; Dhalla, N.S.
Research Communications in Chemical Pathology and Pharmacology 45(2): 297-300
1984
ISSN/ISBN: 0034-5164 PMID: 6148777 Document Number: 233171
Sarcolemmal Ca2+/Mg2+ ATPase was inhibited only about 30% by myosin antiserum that decreased myofibrillar ATPase activity by about 80%. There was a remarkable difference in the effect of myosin antiserum on sarcolemmal Ca2+/Mg2+ ATPase and myofibrillar ATPase with regard to its pre-incubation time with these organelles. Tryptic digestion of the sarcolemmal membrane did not show any change in its inhibitory effect of the myosin antiserum on Ca2+ ATPase. The data distinguish the Ca2+/Mg2+ ATPase from myosin ATPase and suggest that it is an enzyme of the sarcolemmal origin.