Hydroxycotinine metabolism in rabbit liver, lung and kidney tissue homogenates
Rashid Mahmud, A.; Nikolin, A.; Nikolin, B.; Lekić, M.
Arhiv Za Higijenu Rada i Toksikologiju 35(1): 17-23
1984
ISSN/ISBN: 0004-1254 PMID: 6508533 Document Number: 231523
Hydroxycotinine metabolism was investigated in rabbit liver, lung and kidney tissue homogenates, using NADP and NADPH. With NADPH, the liver was the most active organ in metabolizing hydroxycotinine yielding hydroxycotinine-N-oxide (16.3%) as the main metabolic product, along with a little cotinine. With NADP, the formation of cotinine was slightly higher in the kidney and lung tissue incubates than the corresponding formation of cotinine with NADPH. The qualitative identification of the substrate and the metabolic products was carried out by TLC, followed by their quantitative estimation by GLC.