Isolation of phospholipase A1 from Trypanosoma brucei
Hambrey, P.N.; Forsberg, C.M.; Mellors, A.; Tizard, I.R.; Werchola, G.M.
Tropenmedizin und Parasitologie 35(1): 15-19
1984
ISSN/ISBN: 0303-4208 PMID: 6710597 Document Number: 230672
Phospholipase A1 from T. brucei brucei was purified 380-fold by column chromatography on phosphatidylcholine-Sepharose affinity columns followed by DEAE-cellulose anion-exchange chromatography and Sephacryl S-200 molecular exclusion chromatography. Octyl-Agarose hydrophobic column chromatography can be substituted for the PC-Sepharose column. The molecular weight of trypanosomal PLase A1 was found to be 26 000 by SDS-polyacrylamide gel electrophoresis.
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