Blocking activity of the serum from semiallogeneic chimaeras produced by means of cyclophosphamide
Nagurskaya, E.V.; Chernyakhovskaya, I.J.; Fontalin, L.N.
Folia Biologica 30(4): 209-220
1984
ISSN/ISBN: 0015-5500 PMID: 6383881 Document Number: 229386
Blocking activity of the serum from semiallogeneic chimeras produced by the i.p. injection of cyclophosphamide, followed by the i.v. injection of 108 spleen cells from (CBA .times. C57BL/6)F1 hybrid mice into CBA mice, was investigated. The chimera serum inhibited local GVHR [graft vs. host reaction] when injected together with lymphocytes from CBA mice into (CBA .times. C57BL/6)F1 hybrid mice, but not (CBA .times. BALB/c)F1 hybrids. Previous absorption of the chimera serum with C57BL/6 lymphocytes reduced its blocking activity in local GVHR. Absorption with activated CBA anti-C57BL/6 lymphocytes had no effect on blocking activity of the serum. In some experiments the chimera serum inhibited cytotoxic activity of CBA anti-C57BL/6 and BALB/c anti-C57BL/6 lymphocytes on the corresponding target cells in vitro. Graft-vs.-graft reaction of CBA thymocytes against spleen cells from (CBA .times. C57BL/6)F1 hybrid mice was considerably reduced when irradiated chimeras were the recipients. I.v. injection of the chimera serum into the intact CBA mice did not prolong the survival of C57BL/6 skin allografts. It is concluded that the serum blocking factor is an antibody to the transplantation antigen or an antigen-antibody complex.