An improved method for labeling carboxyatractyloside by [3H]KBH4
Ishiyama, S.; Hiraga, K.; Tuboi, S.
Biochemistry International 8(2): 305-311
1984
ISSN/ISBN: 0158-5231 PMID: 6477602 Document Number: 227226
Carboxyatractyloside was labeled with [3H]KBH4 after oxidation of the primary alcohol of the glucose disulfate moiety by dicyclohexylcarbodiimide and P2O5 under anhydrous conditions in a dimethylsulfoxide medium. The 3H-labeled product was purified by DE 52 column chromatography followed by Cellulofine GCL 25 column chromatography. The final 3H-labeled product gave a single spot on a thin-layer chromatogram, and its Rf value was the same as that of authentic carboxyatractyloside. The biological activities (such as inhibition of state 3 respiration and binding to the adenine nucleotide carrier) were also comparable with those of authentic carboxyatractyloside.