Analysis of cytoplasmic aspartate aminotransferase using active enzyme centrifugation

Chebotareva, N.A.; Pavlenko, A.A.; Kurganov, B.I.; Chebotareva, N.A; Pavlenko, A.A; Kurganov, B.I

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Analysis of cytoplasmic aspartate aminotransferase using active enzyme centrifugation

Chebotareva, N.A.; Pavlenko, A.A.; Kurganov, B.I.

Biokhimiia 49(5): 870-874

1984

ISSN/ISBN: 0320-9725

PMID: 6743708

Document Number: 224472

Using active enzyme centrifugation, it was demonstrated that pig heart cytoplasmic aspartate aminotransferase retains its dimeric conformation with a decrease in protein concentration down to 0.2 microgram/ml (50 mM L-aspartate, 5 mM alpha-ketoglutarate, 0.1 M NaCl, 0.05 M potassium-phosphate buffer, pH 7.4, 20 degrees C).