Trihydroxytetraenes: a novel series of compounds formed from arachidonic acid in human leukocytes
Serhan, C.N.; Hamberg, M.; Samuelsson, B.
Biochemical and Biophysical Research Communications 118(3): 943-949
1984
ISSN/ISBN: 0006-291X PMID: 6422933 Document Number: 222543
Addition of 15L-hydroperoxy-5,8,11,13-eicosatetraenoic acid (15-HPETE) to human leukocytes led to the formaton of a novel series of compounds containing 4 conjugated double bonds. The yield of tetraenes was increased .apprx. 100-fold when ionophore A23187 (5 .mu.M) was added simultaneously with 15-HPETE. The structure of the major tetraene was established by physical methods as well as by chemical degradation and found to be 5,6,15L-trihydroxy-7,9,11,13-eicosatetraenoic acid.