Cryodamage on sperm chromatin according to different freezing methods, assessed by AO test

Petyim, S.; Choavaratana, R.

Journal of the Medical Association of Thailand 89(3): 306-313

2006


ISSN/ISBN: 0125-2208
PMID: 16696413
Document Number: 2209
Objective: To evaluate cryodamage effects on human sperm chromatin, motility and cryosurvival rate after freeze-thawing, compared between liquid nitrogen vapour and computerized program freezer assessed by acridine orange staining method (AO test).Material and Method. Fifty semen samples were used After semen analysis, each semen sample was mixed with cryoprotective media and divided into 2 straws. The first straw was frozen with liquid nitrogen vapour and the second with computerized program freezer After 1 month of cryostorage, semen samples were thawed. Sperm chromatin integrity motility, morphology, vitality and sperm cryosurvival rate were determined.Results: DNA damage was significantly greater (p < 0. 001) following freezing with liquid nitrogen vapour than with computerized program freezer Furthermore, the computerized program freezing method significantly provided superior post-thaw sperm motility, vitality and cryosurvival rate, compared with the liquid nitrogen vapour freezing method.Conclusion: Computerized program freezing is recommended as a first choice method for routine cryostorage.

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Cryodamage on sperm chromatin according to different freezing methods, assessed by AO test