Activation of glucose isomerase by magnesium and cobalt ions
Ananichev, A.V.; Ulezlo, I.V.; Egorov, A.M.; Rezchikov, A.A.; Bezborodov, A.M.
Biokhimiia 48(1): 111-118
1983
ISSN/ISBN: 0320-9725 PMID: 6830909 Document Number: 220690
The mode of activating effect of Mg2+ and Co2+ on glucose isomerase from Actinomyces [Streptomyces] olivocinereus 154 was studied and the main kinetic constants were calculated. The activating effect of Mg2+ is of the non-competitive type, that of Co2+ is of the synergistic type. Simultaneous application of optimal concentrations of these cations results in the enzyme activation of a mixed type. Mg2+ produces a stronger activating effect than Co2+, but the latter plays a key role in the enzyme protection against heat inactivation.