Isolation from a pregnant woman of a serum protein responsible for inhibiting an in vitro cytotoxicity reaction
Piquet, Y.; Fizet, D.; Bousquet, J.; Platel, D.; Cabantous, F.; Dubecq, J.P.; Moulinier, J.
Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 177(2): 175-182
1983
ISSN/ISBN: 0037-9026 PMID: 6224538 Document Number: 217058
A non specific immunosupressive factor able to block an in vitro cytotoxicity reaction is demonstrated in the serum of pregnant women. A solution containing this blocking factor is obtained by gel filtration and precipitation of plasma by polyethylene glycol 4 000. Then after immunisation of rabbits the immune serum can be used for affinity chromatography. An alpha 2 glycoprotein has been separated which inhibits the in vitro cytotoxicity reaction and whose molecular weight determined by gel filtration and polyacrylamide gel is about 200 000 daltons.