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Evaluation of an enzymic procedure for the measurement of acetaminophen

Price, C.P.; Hammond, P.M.; Scawen, M.D.

Clinical Chemistry 29(2): 358-361

1983

ISSN/ISBN: 0009-9147
PMID: 6821946
Document Number: 214810
We evaluated a new method for measurement of acetaminophen. From acetaminophen, acetate and p-aminophenol are enzymically produced, and the latter product is determined colorimetrically by reaction with o-cresol and ammoniacal copper sulfate. The method is rapid and precise, and results compare well with those by spectrophotometric and liquid-chromatographic techniques. Major metabolites of acetaminophen, some commonly used drugs, or phenolic substances that may be present in patients with liver or renal disease do not interfere.

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