Evaluation of a new measurement procedure for the concentration of ferritin in serum
Palencia-Domínguez, T.; Martínez-Cervera, J.M.; Fuentes-Arderiu, X.
European Journal of Clinical Chemistry and Clinical Biochemistry 35(2): 117-120
1997
ISSN/ISBN: 0939-4974 PMID: 9056755 Document Number: 477015
The aim of this study was the evaluation of an immunoturbidimetric measurement procedure for the concentration of ferritin in serum, based on the new Tina-quant Ferritin reagents kit (Boehringer Mannheim) in which the antibody-coated latex particles have been modified with regard to the previous Tina-quant Ferritin reagents kit. The evaluation was carried out using the BM/Hitachi 917 Analyzer. The evaluation included the within-run and between-day imprecisions estimation, the comparison of measurement procedures and the assessment of the measuring range. An additional study of between-day imprecision was done using 4 microl and 20 microl of sample. Detection limit was studied using BM/Hitachi 917 (4 microl and 20 microl of sample), ES 300 and Cobas-Core analyzers. The coefficients of variation obtained in the between-day imprecision study are lower when 20 microl of sample is used instead of 4 microl. In the measurement procedure using 20 microl, the coefficient of variation observed at physiological concentrations is 2.4%. The detection limit with 20 microl of sample is lower than with 4 microl. Therefore, in ferropenic anaemia studies it is convenient to use the measurement procedure with 20 microl sample volumes, since it possesses the best metrological characteristics for this purpose.