Sensitivity of preincubated lymphocytes to suppressor regulation
Kalmár, L.; Gergely, P.; Nékám, K.; Gonzalez, R.
Acta Medica Hungarica 40(4): 195-202
1983
ISSN/ISBN: 0236-5286 PMID: 6232498 Document Number: 214689
DNA synthesis of human peripheral blood lymphocytes increases if Con A [concanavalin A] is added to the culture after 24 h preincubation at 37.degree. C. During preincubation the mitogen reactive lymphocytes lose their sensitivity to the suppressive effect of autologous mitomycin-treated mononuclear leukocytes, and of supernatants of autologous preincubated cells. The reactive lymphocytes preincubated at 4.degree. C retain their sensitivity to the suppressive effect of regulatory cells and their supernatants. It is assumed that the enhancement of mitogen response after preincubation at 37.degree. C is caused by a decrease of suppressor regulation of human lymphocytes. Prostaglandins may be regarded as one of the mediators of the suppression.