Activation of Hageman factor (factor XII) by sulfatides and other agents in the absence of plasma proteases
España, F.; Ratnoff, O.D.
Journal of Laboratory and Clinical Medicine 102(1): 31-45
1983
ISSN/ISBN: 0022-2143 PMID: 6343536 Document Number: 208929
Incubation of purified human HF (factor XII) with sulfatides, EA did not influence the results. The presence of albumin greatly enhanced activation of the amidolytic properties of purified HF by EA, even when albumin had been lipid-extracted or treated with DFP or SBTI; albumin also increased activation of HF by sulfatides. Internal cleavage and minimal scission of the HF molecule accompanied the generation of amidolytic properties in mixtures of HF and sulfatides; cleavage was not blocked by SBTI. Negatively charged substances apparently can activate HF in the absence of other enzymes and this activation is accompanied by formation of a 2-chain species of HF.