Prolylendopeptidase activity in various animal organs
Alekseenko, L.P.; Zolotov, N.N.; Pozdnev, V.F.; Orekhovich, V.N.
Biulleten' Eksperimental'noi Biologii i Meditsiny 96(11): 29-32
1983
ISSN/ISBN: 0365-9615 PMID: 6357307 Document Number: 208550
The paper is concerned with the distribution of prolylendopeptidase activity as measured by the hydrolysis of Z-Gly-Pro-pNA in extracts of various cattle tissues (hypophysis, liver, kidneys, heart, skeletal muscle, lungs, spleen, testicles, pancreas, thyroid, brain, small intestine, blood plasma). The highest specific activity was seen in the cerebral cortex, the lowest, in the spleen. The molecular weights of proteins with prolylendopeptidase activity in the extracts of the adenohypophysis, liver, kidneys, skeletal muscle and spleen were determined by gel filtration in an Ultrogel AcA 44 column, and the values of 57 000-66 000 dalton were obtained. Studies of the action of various inhibitors showed that complete inhibition of activity by PCMB (1 X 10(-3) M) was attained only in adenohypophyseal extracts, whereas in those of the spleen, skeletal muscle and liver, the inhibition reached 85%. The activity in the kidneys was inhibited by 65%.