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Optimalized conditions of tick-borne encephalitis virus production in vitro

Slávik, I.; Ciampor, F.; Mayer, V.

Acta Virologica 27(2): 97-104

1983

ISSN/ISBN: 0001-723X
PMID: 6135340
Document Number: 199453
Conditions of the efficient production of tick-borne encephalitis (TBE) virus in chick embryo cell (CEC) monolayer cultures were investigated. The prerequisites of high recovery of the produced virus appeared to be: the use of dense cell monolayers, reasonable frequency of harvests and a suitable buffering of nutrient media. Using low input multiplicity of infection, the peak of the serum-free virus production was observed between 24 and 48 hr post infection (p.i.). The procedure elaborated was useful for isotopic labelling of the virus.

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