Torsional stress induces left-handed helical stretches in DNA of natural base sequence: circular dichroism and antibody binding
Di Capua, E.; Stasiak, A.; Koller, T.; Brahms, S.; Thomae, R.; Pohl, F.M.
EMBO Journal 2(9): 1531-1535
1983
ISSN/ISBN: 0261-4189 PMID: 11892807 Document Number: 199423
Above a threshold of torsional stress, the c.d. spectrum of covalently closed circular DNA of natural base sequence acquires a Z-like contribution and antibodies raised against Z-DNA are bound. Mapping of the antibody binding sites by electron microscopy reveals sites which correlate with stretches enriched in alternating purine-pyrimidine sequences and GC base pairs.