New algorithm for analysis of data obtained by means of circular dichroism titration method
Stavreva, N.; Ruseva, V.; Michailova, D.; Stavrev, P.
Arzneimittel-Forschung 43(1): 73-75
1993
ISSN/ISBN: 0004-4172 PMID: 8447853 Document Number: 421047
A new method for analysis of circular dichroism titration data used for drug-protein binding investigations is proposed. A square equation between molar ellipticity change and total drug concentration is obtained which is examined analytically. A new minimization algorithm for determining the binding sites and association constants of each type of drug-protein complexes is applied.