Effect of biologically active substances on the alkaline phosphatase activity, viability and fertility of bull spermatozoa
Zakhariev, Z.; Iotova, M.; Kichev, G.; Marinov, M.F.; Al-Hanak, H.
Veterinarno-Meditsinski Nauki 20(2): 27-32
1983
ISSN/ISBN: 0324-1068 PMID: 6880016 Document Number: 197377
Studied was the effect of beta-glucuronidase, hydrocortisone, and mercaptoethanol in bull semen on the activity of the alkaline phosphatase and the viability and fertilizing capacity of spermatozoa. It was found that the beta-glucuronidase enzyme in conc. of 270 UI per cu. cm of semen enhanced the activity of one of the forms of AP in agar electrophoresis (AP-1) with the simultaneous enhancement of the thermal resistance of spermatozoa at 46 degrees C and their fertilizing capacity by 9.6 per cent at first insemination. At minimum concentration hydrocortisone (1 X 10(-6) M) lowered the heat resistance of spermatozoa at 39 degrees C. Mercaptoethanol was found to lower by 3 per cent the activity of semen alkaline phosphatase. It is suggested to use the beta-glucuronidase enzyme in the practice of artificial insemination out of all other biologically active agents studied.