Complexity of liver mRNA from normal and thioacetamide-treated rats
Wagiel, B.; Szala, S.; Chorazy, M.
Acta Biochimica Polonica 29(1-2): 111-119
1982
ISSN/ISBN: 0001-527X PMID: 6184921 Document Number: 196352
The complexity and diversity of polysomal poly(A)-containing RNA (mRNA) isolated from livers of normal and thioacetamide (TAA)-treated rats were compared by mRNA/complementary DNA hybridization technique. Three kinetic components (frequency classes) representing in normal mRNA about: I-34, II-32 and III-34% of RNA, apparently contained about 3, 60 and 3400 different sequences of 1 kb each, respectively. The corresponding values for mRNA from TAA-treated rats were about: I-24, II-36, and III-40%, comprising about 1, 30 and 3200 different sequences. It appeared that abundant mRNA sequences (from components I and II) may be 2-5 times more frequently represented in mRNA from TAA-treated than from normal rats. About 15% of mRNA sequences in TAA-treated rats were apparently transcribed from repetitive DNA reassociated to a Cot of 100. The results suggested that thioacetamide, a potent hepatocarcinogen, modulates the transcription or post-transcription regulatory steps of a few selected mRNA species, leading to their accumulation in the cell.