The fate of pancreatic secretory proteins in the blood circulation of dogs and rats
Papp, M.; Varga, G.; Folly, G.; Törcsvári, V.
Acta Physiologica Academiae Scientiarum Hungaricae 59(4): 329-339
1982
ISSN/ISBN: 0001-6756 PMID: 6189364 Document Number: 193973
To follow the fate of intravascular pancreatic secretory proteins, fresh pancreatic juice was collected from anesthetized donor dogs and rats following stimulation of the pancreas by 4 U/kg body wt of secretin + CCK-PZ [cholecystokinin-pancreozymin]. Protein level, lipase and amylase activity of the juice were determined, then the juice was injected into the blood stream of anesthetized animals of the same species. Space of distribution, biological half-life, excretion in urine and bile, secretion in pancreatic juice and activity in thoracic duct lymph of the injected pancreatic secretory proteins were determined. Plasma volume of the recipient animals was also determined. The space of distribution of both lipase and amylase, and that of lipase in dogs was restricted to the plasma volume. In the dogs, the biological half-life of amylase was longer than that of lipase. The excretion of enzymes in the bile of dogs and rats was negligible, while in the urine of rats it reached 10% of the injected amount. Pancreatic juice injected into the blood stream increased both lipase and amylase activities in the pancreatic juice of dogs, and the protein level and output in the pancreatic juice of both dogs and rats. A part of the enzymes which had left the blood circulation was recirculated by the thoracic duct lymph into the blood.