A simple and rapid immunoenzymatic assay of prostatic acid phosphatase in serum
Paoli, C.; Bardelli, F.; Tarli, P.; Neri, P.
Annales de Biologie Clinique 40(2): 75-78
1982
ISSN/ISBN: 0003-3898 PMID: 6751168 Document Number: 193538
A method is described for the quantitative assay of human prostatic acid phosphatase after its isolation from serum by a double antibody technique employing a specific rabbit antiserum and a goat anti-rabbit gammaglobulin serum. After centrifugation, the precipitate is analyzed for phosphatase activity using p-nitrophenylphosphate as substrate. The method allows a rapid, precise, specific and sensitive assay.