Inhibition of basophil histamine release by anti-inflammatory steroids. II. Studies on the mechanism of action
Schleimer, R.P.; MacGlashan, D.W.; Gillespie, E.; Lichtenstein, L.M.
Journal of Immunology 129(4): 1632-1636
1982
ISSN/ISBN: 0022-1767 PMID: 6180017 Document Number: 192882
The glucocorticosteroids inhibit the IgE-dependent release of histamine by human basophils with an order of potency that very closely parallels that found in vivo (i.e., triamcinolone acetonide > dexamethasone > betamethasone > prednisolone > hydrocortisone .mchgt. progesterone .apprx. tetrahydrocortisone .apprx. 0). The effect is seen after a 24-h preincubation with nanomolar to micromolar concentrations of glucocorticoid. Release of histamine stimulated by the formyl methionine containing peptide f-Met-Leu-Phe, the Ca ionophore A23187 -dependent stimuli f-Met-Leu-Phe, A23187 and TPA, the inactivation of IgE-dependent histamine release by glucocorticoids may not be the result of PLA2 inhibition.