Mucosal mast cells. I. Isolation and functional characteristics of rat intestinal mast cells

Befus, A.D.; Pearce, F.L.; Gauldie, J.; Horsewood, P.; Bienenstock, J.

Journal of Immunology 128(6): 2475-2480

1982


ISSN/ISBN: 0022-1767
PMID: 6176638
Document Number: 192559
A procedure was developed for the dispersion of mast cells from the intestinal lamina propria (LP) and epithelium of rats infected with the intestinal nematode Nippostrongylus brasiliensis. The dispersed cells were morphologically and histochemically similar to intestinal mucosal mast cells (MMC) in situ and were distinguishable from peritoneal mast cells (PMC). MMC derived from the LP or epithelium of parasitized animals secreted histamine in response to the specific parasite antigens as well as anti-IgE. Unlike PMC, these cells were unresponsive to the basic secretagogues 48/80 and bee venom peptide 401. Similarly, bee venom peptide 401 conjugated with dansyl chloride binds to PMC and mast cells in the thymus and intestinal serosa, but not to mast cells in or derived from the intestinal LP and epithelium. Studies on PMC treated by the intestinal cell isolation procedure show that the functional characteristics of the MMC cannot be solely attributed to the isolation procedure. Isolated MMC were morphologically, histochemically and functionally different from PMC, as suggested by previous in vivo studies of the normal intestine.

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