Comparative effects of methotrexate, two nonclassic folic acid antagonists, and cytarabine on hematopoietic precursor cells
Marsh, J.C.
Cancer Treatment Reports 66(3): 499-504
1982
ISSN/ISBN: 0361-5960 PMID: 6460555 Document Number: 190607
The effects of three antifolates, methotrexate (MTX), triazinate (TZT), and a trimethoxyquinazoline derivative (TMQ), and of cytarabine (ara-C) on leukocyte-committed colony-forming cells from three species were compared using an assay allowing in vivo metabolism in an agar diffusion chamber. Dose-survival curves approximated plateaus, and the level of the plateau was variable among species. Canine marrow colony-forming cells were less sensitive to MTX (about 90% survival) than those from mice or man (25%-40% survival). Human marrow colony-forming cells were less sensitive (70% survival) to TZT than those from mice or dogs (25% survival). No interspecies differences were found for TMQ or ara-C. Mouse marrow in situ was less sensitive to iv MTX than mouse marrow in the chamber. No such difference was found for iv TMQ or ara-C. This comparison could not be made for ip TZT.