Sequential isolation of the structural components of the cell wall of Streptococcus group a
Blinnikova, E.I.; Shchelkova, N.S.; Savel'ev, E.P.; Paterov, G.I.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 7: 41-46
1982
ISSN/ISBN: 0372-9311 PMID: 6812328 Document Number: 190393
The scheme of the consecutive isolation of cell-wall components from group A streptococci, type 29, has been developed. Extraction by means of 1 M hydroxylamine buffer with pH 6.0 (stage 1) and 10.0 (stage II) has yielded proteins showing different behavior in the double immunodiffusion test in agar. The protein obtained at pH 6.0 has given cross reaction with antiserum to type 1, while the protein obtained at pH 10.0 has been found to react only with homologous serum to type 29. Extraction with 10% trichloroacetic acid at 4 degrees C (stage III) and 5% trichloroacetic acid (stage IV) has resulted in obtaining, respectively, polysaccharide forming one line with group-specific antiserum to group A in the double immunodiffusion test and polysaccharide having no such activity. At the last stages the HCl extract of the cell-wall residue (stage V) and peptidoglycan (stage VI) have been obtained.