Preliminary chemical and X-ray studies on the interactions of E. coli DNA with putrescine
Takeda, Y.; Nara, H.; Hayami, M.; Iwahashi, K.; Mitsui, Y.
Nucleic Acids Symposium Series 11: 181-184
1982
ISSN/ISBN: 0261-3166 PMID: 6763978 Document Number: 189772
The interactions of E.coli DNA with putrescine in aqueous solution and fiber were studied by melting temperature analysis, equilibrium dialysis and X-ray diffraction. The equilibrium binding of putrescine to DNA is shown, of which association constant is in the order of 10(3) 1/mol. The binding of putrescine stabilizes the B-form of DNA fiber and the crossbridge of putrescine in the intra-double helix is suggested by precipitate formation experiments of various polyamines with DNA.