Top Abstract Purchase Related
Home
>
Node
>
Subnode

Determination of the catalytic activity of lipase with trilinolein as substrate

Rick, W.; Hockeborn, M.

Journal of Clinical Chemistry and Clinical Biochemistry 20(10): 745-752

1982

ISSN/ISBN: 0340-076X
PMID: 6815298
Document Number: 184436
The applicability of the two point test reported by Proelss & Wright ((1977) Clin. Chem. 23, 522-531) for the determination of lipase was investigated. The substrate, trilinolein, is indeed cleaved by pancreatic lipase without interference from liver esterase or cholinesterase; on the other hand, the reported colour reaction gives nonreproducible results. Furthermore, the continuous UV-test showed a lag phase, the duration of which depends on the added catalytic activity. For these reasons the method of Proelss & Wright for the determination of lipase in serum does not give diagnostically useful results.

Document emailed within 1 workday
Secure & encrypted payments

Shimayama, T.; Sawata, S.; Komiyama, M.; Takagi, Y.; Tanaka, Y.; Wada, A.; Sugimoto, N.; Rossi, J.J.; Nishikawa, F.; Nishikawa, S. 1992: Substitution of non-catalytic stem and loop regions of hammerhead ribozyme with DNA counterparts only increases KM without sacrificing the catalytic step (kcat): a way to improve substrate-specificity Nucleic Acids Symposium Series 27: 17-18
Tsyganov, E.P.; Shaternikov, V.A. 1978: Method of determining lipase activity using a triglyceride suspension adsorbed on a solid carrier as substrate Laboratornoe Delo 4: 221-222
Stresser, D.M.; Blanchard, A.P.; Turner, S.D.; Erve, J.C.; Dandeneau, A.A.; Miller, V.P.; Crespi, C.L. 2000: Substrate-dependent modulation of CYP3A4 catalytic activity: analysis of 27 test compounds with four fluorometric substrates Drug Metabolism and Disposition: the Biological Fate of Chemicals 28(12): 1440-1448
Kiselev, P.A.; Garda, G.; Finch, S.A.; Stir, A.; Khatyleva, S.Iu.; Akhrem, A.A. 1990: Regulation of the catalytic activity of the monooxygenase enzyme system depending of the substrate structure and phospholipid composition of the model membrane Biokhimiia 55(11): 2058-2071
Stein, W.; Liebich, H.M.; Locher, M.; Mildner, A.; Ludwig, U. 1979: Evaluation of the aca lipase (DuPont) determination and of the lipase determination according to Myrick (Calbiochem-Behring) Journal of Clinical Chemistry and Clinical Biochemistry 17(10): 653-656
Lee, E.; Miki, Y.; Yoshida, T.; Tsuruoka, Y.; Kariya, K. 1989: Bone marrow peroxidase in mice: determination of the level and the catalytic activity Research Communications in Chemical Pathology and Pharmacology 66(1): 135-143
Boger, M.M.; Kornilova, V.A.; Dykul', N.M. 1973: Method of determination of the activity of blood lipase and duodenal contents Laboratornoe Delo 8: 480-482
Fruchart, J.C.; Sezille, G.; Jaillard, J. 1974: New method of determination of the lipase activity of the serum. Clinical applications Lille Medical: Journal de la Faculte de Medecine et de Pharmacie de l'Universite de Lille 19(2): 87-90
Iakovlev, V.A.; Levchuk, T.P.; Malakhova, E.A. 1976: Use of potentiometric titration at constant pH for determination of the activity of lipoprotein lipase Voprosy Meditsinskoi Khimii 22(3): 417-421
Khramov, V.A. 1977: Substrate for determination of agmatinase activity in bacteria Laboratornoe Delo 10: 628-630
Antonov, M.P.; Antonova, L.A.; Laputina, T.V. 1985: Determination of ceruloplasmin activity using p-phenylenediamine as substrate Laboratornoe Delo 1985(6): 335-338
Belozorov, A.P. 1977: Method of substrate isolation for determination of beta-glucuronidase activity Laboratornoe Delo 11: 691-692
Apostolova, I.S.; Kiriakov, A.K. 1983: Changes in the postheparin plasma activity of lipoprotein lipase and liver triglyceride lipase in 2 age groups of rabbits Eksperimentalna Meditsina i Morfologiia 22(2): 97-101
Wang, Y.; Pallen, C.J. 1991: The receptor-like protein tyrosine phosphatase HPTP alpha has two active catalytic domains with distinct substrate specificities EMBO Journal 10(11): 3231-3237
Zheng, D.Q.; Shurafa, M.; James, H.L. 1996: Factor VII G331D: a variant molecule involving replacement of a residue in the substrate-binding region of the catalytic domain Blood Coagulation and Fibrinolysis: An International Journal in Haemostasis and Thrombosis 7(1): 93-96
Han, Z.-h.; Yu, K.-p. 2012: Determination of β-naphthol in the air of workplace by catalytic spectrophotometric Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 30(7): 534-535
Sadowska, J. 1992: Ribonuclease P--an example of catalytic RNA activity Postepy Biochemii 38(3): 112-122
Sitniewski, S.; Grodzka, M. 1980: Units of catalytic quantity and catalytic concentration of the international SI unit system in clinical enzymology Wiadomosci Lekarskie 33(14): 1133-1139
Ventrucci, M.; Pezzilli, R.; Garulli, L.; Liguori, L.; Moratti, R.; Melzi d'Eril, G.V. 1994: Clinical evaluation of a new rapid assay for serum lipase determination Italian Journal of Gastroenterology 26(3): 132-136
Odai, O.; Sakata, T.; Orita, M.; Hiroaki, H.; Uesugi, S.; Tanaka, T. 1989: Synthesis and properties of RNA with catalytic activity Nucleic Acids Symposium Series 21: 105-106