Inhibition of hepatic microsomal mixed function oxidase system during acute cadmium toxicity in rats, mice, guinea pigs & rabbits
Soni, M.G.; Govindwar, S.P.; Desai, B.J.; Gawai, K.R.; Zubairy, Y.K.; Kachole, M.S.
Indian Journal of Experimental Biology 20(8): 600-602
1982
ISSN/ISBN: 0019-5189 PMID: 7173992 Document Number: 183748
Drug metabolizing reactions of hepatic microsomal cytochrome P450 were studied during acute Cd toxicity in rats, mice, guinea pigs and rabbits. The activities of aminopyrine N-demethylase, acetanilide hydroxylase, NADPH-cytochrome c reductase and the concentrations of cytochrome P450, cytochrome b5 and total heme were investigated. Hepatic microsomal mixed function oxidase system exhibited a clear and differential inhibitory action of Cd acetate (2 mg Cd2+/kg for rats and mice, 1 mg Cd2+/kg for guinea pigs and rabbits, i.p. single dose). Rats showed higher inhibition in all cases. Acetanilide hydroxylase activity was remarkably inhibited in females. Female mice showed a significant decrease in microsomal protein content without any change in mixed function oxidase system. Comparison on the basis of per gram liver showed that cytochrome P450 was the major target component of the system responsible for the inhibition due to Cd.