Reproduction of vaccine and virulent Rickectsia prow azeki strains in continuous cell lines at different temperatures
Gudima, O.S.
Acta Virologica 26(5): 390-394
1982
ISSN/ISBN: 0001-723X PMID: 6128907 Document Number: 183415
The features of intracellular development of the virulent Breinl strain and 3 vaccine E strains of R. prowazeki have been followed in continuous [Murine Friend leukemia] FL, McCoy and B cell cultures at temperatures of 30.degree., 35.degree., 37.degree., 38.5.degree. and 40.degree. C. The virulent Breinl strain multiplied well at these temperatures in McCoy and B cells but it had been gradually lost when cultured at 40.degree. C in FL cells. In contrast to the virulent Breinl strain the vaccine E strains have lost their capacity of long term reproduction at 38.5.degree. C. At 40.degree. C the E strains did not multiply in and had been eliminated from the McCoy and B cells; thus the vaccine E strain revealed a ts[temperature-sensitive]-phenotype and was found to represent a ts-mutant.