Assay of genomic DNA homology among strains of different virulent leptospira by DNA hybridization
Huang, N.; Dai, B.
Hua Xi Yi Ke da Xue Xue Bao 23(2): 122-125
1992
ISSN/ISBN: 0257-7712 PMID: 1452138 Document Number: 393309
Nick translation labelling technique was applied to the preparation of two 32P labelled genomic DNA probes from L. interrogans serovar Lai strain 017 and Leptonema illini strain 3055, respectively. Dot-blotting and southern-blotting with DNA of five strains leptospira from different genus and species were performed. The results showed that certain differences among the L. interrogans, L. biflexa and Leptonema illini could be detected by endonuclease assay, L. interrogans strain 017 with L. biflexia strain Patoc I and Leptonema illini 3055 strain exhibited very little homology. L. interrogans strains 017, 601, and 245 from different serogroup and serovar had a high degree of homology. Leptonema illini strain 3055 showed little homology with L. interrogans and L. biflexa. Therefore DNA hybridization may be used as a tool for the identification and classification of leptospira.