Purine nucleoside phosphorylase activity in T-enriched preparations of human peripheral lymphocytes

Russo, M.; Pizzella, T.; Nardiello, S.; Galanti, B.

Bollettino della Societa Italiana di Biologia Sperimentale 57(9): 990-992

1981


ISSN/ISBN: 0037-8771
PMID: 6793048
Document Number: 180726
Purine nucleoside phosphorylase (PNP) activity was measured in peripheral blood mononuclear cells and in T-enriched populations from 6 normal subjects. PNP activity in T-enriched populations were in average 50% of that in unseparated mononuclear cells. In our cell preparations, T-cells appeared to contribute to 27-49% of total biochemical PNP levels in peripheral blood mononuclear cells. T-lymphocytes obtained by rosetting with sheep red blood cells could be a suitable cell population for determining PNP activity in patients with immune disfunction.

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