Reversed-phase supports for th resolution of large denatured protein fragments
Pearson, J.D.; Mahoney, W.C.; Hermodson, M.A.; Regnier, F.E.
Journal of Chromatography 207(3): 325-332
1981
ISSN/ISBN: 0021-9673 PMID: 7228938 Document Number: 179287
Large pore (.gtoreq. 300 .ANG.), spherical particle silica stationary phases possessing C18 or C8 hydrocarbon ligands out-performed small pore (60-100 .ANG.), irregularly shaped silicas for the purification of large denatured peptides . Since columns 5 cm in length appeared to be as effective in separating peptides as columns 5 times longer, it is likely that large peptides absorb to the matrix rather than partition between the stationary and mobile phases.