Freeing rabies virus of the neuroallergenic factor from brain tissue
Karakuyumchan, M.K.; Nadaichik, L.V.; Pille, E.R.; Rozina, E.E.
Acta Virologica 25(3): 155-158
1981
ISSN/ISBN: 0001-723X PMID: 6115564 Document Number: 177400
A method of fixed rabies virus purification from infected sheep brains was proposed. It consisted of suspending the brain tissue in phosphate-buffered hypertonic (0.3 M) NaCl solution, shaking at 37 degrees C and low speed centrifugation at the same temperature. From 65 to 85% ballast proteins were removed, the neuroallergenic activity of the material was lost, but practically no losses of the virus occurred.
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