T-Cell hydrids: I. Selection of thymidine kinase deficient subline EL-4R from EL-4 cell population
Bubeník, J.; Símová, J.; Kára, J.; Kypĕnová, H.; Bubeníková, D.
Folia Biologica 27(3): 209-216
1981
ISSN/ISBN: 0015-5500 PMID: 6973493 Document Number: 176016
Thymidine kinase deficient (TK-) cell line EL-4R suitable for production of T cell hybrids was selected from the [mouse dimethylbenzanthracene-induced leukemia] EL-4 cell population by treatment with 5-bromodeoxyuridine. Comparison of the EL-4 and EL-4R cell lines revealed differences in chromosome constitution and electrophoretic mobility of both cell populations. Both EL-4 and EL-4R cells had a modal number of 39 chromosomes; the percentage of cells with modal number of chromosomes and the percentage of biarmed chromosomes in the EL-4R line was higher than that of the EL-4 line. Anodic electrophoretic mobility of EL-4R cell population was faster than that of EL-4 cells, the respective mean values being 0.96 and 0.91 .times. 10-4 cm2 s-1 V-1.