Water radiolysis products and nucleotide damage in gamma-irradiated DNA
Feldberg, R.S.; Carew, J.A.
International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine 40(1): 11-17
1981
ISSN/ISBN: 0020-7616 PMID: 6266972 Document Number: 175141
The radiation chemistry of nucleotide damage introduction into DNA gamma-irradiated in dilute aqueous solution has been studied with a damage-specific DNA binding protein. Irradiation in air, N2 or N2O in the presence and absence of free radical scavengers revealed that protein-recognizable lesions were introduced both by the hydrated electron and by the combination of the hydroxyl radical and superoxide anion radical, but not by the hydroxyl radical alone. In addition, nucleotide damage was introduced into DNA by an enzymatic superoxide-generating system.