Reactive SH-groups in clinical practice. Methodological notes
Ferraccioli, G.F.; Baldini, A.; Ambanelli, U.
Acta Bio-Medica de l'Ateneo Parmense 52(5): 171-177
1981
ISSN/ISBN: 0392-4203 PMID: 6460402 Document Number: 174589
Experimental investigations have been performed in order to point out the extrinsic factors influencing the results of -SH group determination. It has been observed that the anticoagulants normally used to obtain plasma samples, influence the serum -SH groups; in particular, heparin increases and citrate depresses the levels; the most reproducible data were obtained in serum samples. In addition it has been shown that the exposure to light for 6-8 hours lowered the serum -SH groups, whereas darkness prevented these changes. Serum-SH groups were not influenced by the administration of non steroidal anti-inflammatory agents, whereas D-Penicillamine and Tiopronin induced an increase of the -SH levels, ranging between 15-20%. The levels returned to the basal values within two hours from the drug intake. Following the methodological criteria here described it is possible to obtain a good information about the -SH group reserve, which free radicals generated in several diseases could consume. The practical usefulness of the serum -SH group determination in oxidative-peroxidative processes in discussed.