Regulation of genetic activity by thyroid hormones. Effect of purified cytoplasmic hormone-receptor complex on the chromatin template activity
Abdukarimov, A.; Adylova, A.T.; Kkhan, M.Z.
Problemy Endokrinologii 27(1): 50-53
1981
ISSN/ISBN: 0375-9660 PMID: 6262747 Document Number: 173872
This paper continues a work series concerning the investigation of the functional activity of thyroxin receptor isolated from 105000 g fractions of the rat liver cytoplasm. Previously it was shown that the hormone-receptor complex stimulates the RNA-polymerase activity. In this work it was demonstrated that the complex changes the chromatine template activity, determined both under standard conditions and in the absence of transcription reinitiation. It appears that the hormone-receptor complex increases the number of the transcription initiation sites. Thyroxin cytoplasmatic receptor and/or the hormone, tested separately, exert no pronounced activating effect. It was concluded that the primary interaction between the hormone and specific receptor is necessary for participation in the transcription processes.