Evaluation of ion exchange resins and various enzymes in thiamine analysis
Ellefson, W.C.; Richter, E.; Adams, M.; Baillies, N.T.
Journal - Association of Official Analytical Chemists 64(6): 1336-1338
1981
ISSN/ISBN: 0004-5756 PMID: 7309654 Document Number: 172759
Four ion exchange resins and 9 enzyme preparations are evaluated for use in the official AOAC (Association of Official Analytical Chemists) thiamine method because Decalso and Clarase or Mylase P either are no longer available or are available in a form that is not suitable for use in the assay. The enzymes are prepared in the same manner described for Clarase or Mylase P in the AOAC method and are compared with Clarase T300 for their effectiveness in releasing thiamine from thiamine phosphate, and their ability to produce similar results on food samples. Rhozyme S is 90-100% as effective as Clarase T300 in both of these respects. The other enzymes tested were not satisfactory. Further study is necessary because Rhozyme S is no longer manufactured. The ion exchange resins are prepared for use in the manner described for Decalso in the AOAC method. Recoveries of thiamine range from 95 to 100%, using Bio-Rex 70 (hydrogen form) ion exchange resin. The other resins tested were not satisfactory.