Improvements in the laboratory diagnosis of hemorrhagic fever with a renal syndrome
Bugaeva, N.P.; Mikhaĭlov, M.I.; Favorov, M.O.; Ketiladze, E.S.
Voprosy Virusologii 5: 618-621
1981
ISSN/ISBN: 0507-4088 PMID: 6121424 Document Number: 172307
The results of the employment of direct solid-phase enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) for the detection of antigen and antibody to the virus of hemorrhagic fever with renal syndrome (HFRS) are first presented. Unlike the previously used fluorescent antibody technique (FAT), the immunosorbent methods allow the quantitation of te HFRS antigen, the sensitivity of ELISA and RIA methods for antigen detection being 8-16-fold higher than that of FAT. In antibody determinations, a significant advantage of ELISA and RIA consists in reproducibility of the results due to the standardization of the antigen dose. The observed dynamics of antibody response to HFRS virus is peculiar and somewhat similar to seroconversion in epidemic nephropathia in the Scandinavian countries.