Protein and RNA composition of a mild (W) and a severe (P6) strain of cucumber mosaic virus
Roberts, P.L.; Wood, K.R.
Microbios 32(127): 37-45
1981
ISSN/ISBN: 0026-2633 PMID: 7339445 Document Number: 171558
The protein and RNA composition of mild (W) and severe (P6) chlorosis-inducing strain of cucumber mosaic virus (CMV) have been compared. The coat polypeptide of both strains migrated with an apparent molecular weight of 26.9 +/- 0.4 (95%) x 10(3) on SDS polyacrylamide gels. They also co-migrated, and showed no anomaly in SDS binding, on SDS agarose gels. Their amino acid compositions were similar except for small differences in the number of alanine, glycine and proline residues. Analysis by double immuno-diffusion revealed a fast migrating antigen, which showed a reaction of identity between strains, and also a slow migrating antigen which showed a reaction of partial identity. Both CMV-P6 and CMV-W possessed a normal genome complement of RNAs 1 to 4. In addition, an isolate of both strains was found that contained large quantities of satellite RNA 5. However, its presence did not modify symptom severity in tobacco or tomato.