Immunofluorescent studies of the microtubular system in slime moulds
Unger, E.; Cappuccinelli, P.; Rubino, S.; Vater, W.
Gegenbaurs Morphologisches Jahrbuch 127(5): 683-687
1981
ISSN/ISBN: 0016-5840 PMID: 7044885 Document Number: 170756
Using indirect immunofluorescence the microtubule system of slime molds has been investigated with antitubulin- and anti-MAP-antibodies. An extended cytoplasmic microtubule (MT) network with nucleus associated organells as centres can be visualized. The spindle apparatus with some astral fibres, the central spindle and the spindle pole bodies is stained in mitotic cells. In slime molds like in higher eucaryots MTs are sensitive against cold and MT-poison treatment, the MTs are composed of tubulin and MAP's, and tubulin is cross reactive with pig brain tubulin.