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Spectrofluorimetric study of interaction between acetylcholine and brain proteins

Sergienko, N.G.; Luchko, N.A.; Brovina, N.N.

Ukrainskii Biokhimicheskii Zhurnal 53(3): 18-21

1981

ISSN/ISBN: 0201-8470
PMID: 7256947
Document Number: 169511
Interaction of acetyl-choline in 10(-4) . 10(-12) M concentration with a water-soluble fraction of homogenates of different brain areas (medulla oblongata, pons varolii, sensomotor cortex, dorsal and ventral hippocamp, hypothalamus, amygdaloid nuclei region and septal region) was studied by the spectrofluorimetric method. Fluorescence complexes spectra at excitation wavelength of 280, 296 nm were investigated. It is shown that the ACH addition to the water-soluble fractions results in reduction of the spectrum intensity and in insignificant shift of the fluorescence maxima to a short-wave region. This effect is supposed to be due to ACH interaction with the cholinoreceptor (CHR). The number of CHR in all the brain regions studied is calculated. Good correlation is observed between the regional distribution of CHR and the acetylcholinesterase activity.

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Mallol, J.; Rodríguez Farré, E.; Valdecasas, F.G. 1978: Factors that condition the interaction of noradrenaline with soluble proteins of cat brain Archivos de Farmacologia y Toxicologia 4(1): 18-19
Tripathi, H.L.; Vocci, F.J.; Brase, D.A.; Dewey, W.L. 1987: Effects of cannabinoids on levels of acetylcholine and choline and on turnover rate of acetylcholine in various regions of the mouse brain Alcohol and Drug Research 7(5-6): 525-532
Thakur, M.K.; Kanungo, M.S. 1980: Interaction of histones and non-histone chromosomal proteins of the brain of new-born and adult rats with deoxyribonucleic acid Indian Journal of Biochemistry and Biophysics 17(2): 81-84
Sakai, M.; Fukuyama, H.; Sato, K.; Kudoh, I. 2002: Effects of fentanyl on acetylcholine release from hippocampus and righting reflex in rat: an in vivo brain microdialysis study Masui. Japanese Journal of Anesthesiology 51(2): 118-123
Filenko, A.M.; Zuma, V.L.; Bodach, P.H. 1976: Study of interaction between alcohols (mono- and polhydric) and proteins in aqueous solutions Ukrains'kyi Biokhimichnyi Zhurnal 48(1): 89-95
Saito, Y. 1974: The circadian rhythm of brain acetylcholine with reference to its role in the brain function Seishin Shinkeigaku Zasshi 76(3): 193-206
Dambinova, S.A.; Lekomtseva, T.M.; Margulis, M.N.; Ogurtsov, R.P. 1987: Molecular organization of glutamate-sensitive chemo-stimulated nerve cell membranes. Interaction of monoclonal antibodies with glutamate-binding membrane proteins in the rat brain, human neuroblastoma and molluscan neurons Biokhimiia 52(9): 1523-1530
Flouvat, B.; Bazin, M.; Lucsko, M.; Roux, A.; Guedon, J. 1978: Spectrofluorimetric estimation in biological fluids of a new beta-blockader: atenolol. Application to its pharmacokinetic study Annales de Biologie Clinique 36(4): 339-346
Coronel, C.; Ballesteros, M.; del Castillo, B.; Söllhuber, M.M.; Menendez, J.C. 1988: Spectrofluorimetric study of 1,3,4,6,7,11b-hexahydro-9,10-dimethoxy-2H-benzo(a) quinolizine-2-spiro-3'-(1'-phenyl) succinimide hydrochloride Journal de Pharmacie de Belgique 43(1): 37-45
Ansorg, R.; Dames, W.; Neuhoff, V. 1971: Micro-disc electrophoresis of brain proteins. II. Study of different extraction methods Arzneimittel-Forschung 21(5): 699-710
Bolger, M.B.; Dionne, V.; Chrivia, J.; Johnson, D.A.; Taylor, P. 1984: Interaction of a fluorescent acyldicholine with the nicotinic acetylcholine receptor and acetylcholinesterase Molecular Pharmacology 26(1): 57-69
Carpenter, D.O.; Greene, L.A.; Shain, W.; Vogel, Z. 1976: Effects of eserine and neostigmine on the interaction of alpha-bungarotoxin with Aplysia acetylcholine receptors Molecular Pharmacology 12(6): 999-1006
Komissarov, I.V.; Samoilovich, I.M.; Shovtuta, V.I. 1976: Use of parameters of ionic permeability and mechanical activity for the rating of interaction of acetylcholine with the smooth muscle M-cholinoreceptors Farmakologiia i Toksikologiia 39(4): 417-420
Frijns, J.M. 1971: Chromatographic isolation and direct spectrofluorimetric determination on the chromatogram of microgram amounts of drugs. V. Study of liquids, tablets and suppositories containing ergometrine and ergotamine Pharmaceutisch Weekblad 106(47): 865-881
Mendzheritskiĭ, A.M.; Trapezontseva, R.A.; Vilkov, G.A. 1982: Dicarboxylic amino acids in brain proteins under conditions of sensitization with brain antigen Ukrainskii Biokhimicheskii Zhurnal 54(1): 3-7
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Hársing, L.G.; Illés, P.; Fürst, S.; Vizi, E.S.; Knoll, J. 1979: The effect of prostaglandin E1 on acetylcholine release from the cat brain Acta Physiologica Academiae Scientiarum Hungaricae 54(2): 177-185
Horský, J.; Krs, O.; Bielavský, J. 1990: Role of interaction of the tanycytes of the third ventricle of the brain with the vascular bed endothelium in enzymatic activation of the parkinsonism-inducing proneurotoxin MPTP in rat brain Folia Morphologica 38(1): 24-27
Vizi, E.S.; Ono, K.; Adam-Vizi, V.; Duncalf, D.; Földes, F.F. 1984: Presynaptic inhibitory effect of Met-enkephalin on [14C] acetylcholine release from the myenteric plexus and its interaction with muscarinic negative feedback inhibition Journal of Pharmacology and Experimental Therapeutics 230(2): 493-499
Warnick, J.E.; Maleque, M.A.; Albuquerque, E.X. 1984: Interaction of bicyclo-octane analogs of amantadine with ionic channels of the nicotinic acetylcholine receptor and electrically excitable membrane Journal of Pharmacology and Experimental Therapeutics 228(1): 73-79