In vitro assessment of human polymorphonuclear leucocytes collected by two different leucapheresis methods: continuous flow centrifugation and continuous flow filtration
Cardellach, F.; Feliú, E.; Matutes, E.; Grañena, A.; Marín, P.; Martí, M.; Vives-Corrons, J.L.; Rozman, C.
Sangre 26(1): 1-14
1981
ISSN/ISBN: 0036-4355 PMID: 7256499 Document Number: 169216
The function of polymorphonuclear leukocytes (PMN) obtained from 48 healthy human donors was assessed. The PMN were collected through continuous flow centrifugation (CFC) in 20 instances and continuous flow filtration (CFF) in 28 instances. Subjects [44] were used as a control group in assessing the effect of drugs administered prior to leukapheresis (namely, heparin and glucocorticoids). With regard to the control PMN, the CFF-PMN showed decreased chemotaxis on serum (P < 0.05) and zymosan activated serum (P < 0.0005), and depressed random mobility (P < 0.02), engulfment (P < 0.005) and iodination (P < 0.0005). The CFC-PMN had normal behavior in every step of the functional assessment, although NBT[nitro-blue tetrazolium]-reduction dye test showed decreased values (P < 0.01). When comparing the functionalism of PMN collected by both methods between each other, the CFF-PMN showed decreased chemotaxis (P < 0.01) and iodination (P < 0.01). The drugs given to donors prior to leukapheresis enhanced all the PMN functions except their engulfment capability. Filtration procedures apparently decrease the PMN functionalism in virtually all its different stages, as opposed to centrifugation; such effect would not be related to the treatment the donors receive (heparin and glucocorticoids) but to the leukapheresis method itself.