Improvement in the granulocyte harvest from normal donors by means of a combination of "continuous flow" centrifugation and filter leukapheresis
Gmür, J.; Deluigi, J.; Straub, P.W.
Schweizerische Medizinische Wochenschrift 105(47): 1589-1590
1975
ISSN/ISBN: 0036-7672 PMID: 1209225 Document Number: 90978
In 27 healthy donors a total of 30 leukaphreses were performed with the Aminco cellseparator. Pretreatment by steroids, anticoagulation by heparin and addition of fluid gelatin to the input line was performed in all runs. The combination of continuous flow centrifugation and filtration leukapheresis in 9 of the 30 runs resulted in highly significant increase in the yield of neutrophil granulocytes to a mean of 59.6x10(9) as compared with a mean of 30.8x10(9) obtained the conventional continuous-flow centrifugation alone. This significance is valid for the total yield of granulocytes as well as for the yield per hour of run or per liter of blood processed. No adverse reaction occurred.