Cadaver heart preservation and transplantation: an experimental study
Arikawa, K.
Nihon Kyobu Geka Gakkai 28(11): 1643-1653
1980
ISSN/ISBN: 0369-4739 PMID: 7007527 Document Number: 166843
Preservation and succeeding orthotopic allotransplantation of the cadaver heart was studied experimentally. Grafts were obtained from exsanguinated dogs. Preservation model was prepared in the thoracic cavity of the cadaver. Coronary perfusion with diluted autologous blood using a 40-50 cm of gravity infusion into the aortic arch was started after 30 min of ascertained death. Oxygenation of the perfusate by means of bubbling was carried out with 100% O2 into a reservoir. Myocardial temperature fell rapidly to 15.degree. C after the commencement of coronary perfusion supplemented by external bath of ice slush in the pericardial well. Preservation was carried out for 1 h. O2 consumption during preservation remained lower than anticipated. Biochemical data of the perfusate revealed reasonable metabolic condition corresponding with lowered temperature level. The phenomenon certified that blood perfusion of the coronary artery at a given temperature was a useful selection. Of 30 grafts selected randomly, 20 were transplanted orthotopically to the recipients by means of modified Shumway's technique. All grafts restored their cardiac beat after transplantation. Only 5 grafts could sustain recipient's whole circulation without assistance of cardiopulmonary bypass for over 2 h. There were 3 cases with excellent hemodynamic condition which lasted for over 6 h with preserved and transplanted grafts. Coronary perfusion preservation of the cadaver heart using diluted autologous blood with 40-50 cmH2O of gravity pressure apparently was acceptable due to success in functional determination through orthotopic allotransplantation.