Quantitation of serum ferritin by enzyme-linked immunosorbent assay (ELISA)
Conradie, J.D.; Mbhele, B.E.
South African Medical Journal 57(8): 282-287
1980
ISSN/ISBN: 0256-9574 PMID: 6996142 Document Number: 166664
The solid phase consists of rabbit antiferritin immunoglobulin G adsorbed onto microtitre plates. Ferritin from standards or samples binds to the solid phase and is detected by a conjugase consisting of the antiferritin IgG and horseradish peroxidase. The method takes 5 h and is precise. The range is 1 to 64 mu g/litre and serum has to be diluted 10-fold.
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