To the problem of the intervascular volume effect of intravenously administered sorbitol solution 40%
Kleemann, P.P.; Brost, F.; Müller-Suur, N.; Halmágyi, M.
Der Anaesthesist 29(12): 642-649
1980
ISSN/ISBN: 0003-2417 PMID: 7212245 Document Number: 163903
Despite the clinical importance of changes in circulating blood volume caused by the infusion of osmotherapeutics, the necessity to verify those factors responsible for contradictory or incorrect results on blood volume changes by infusion of osmotherapeutics published in literature was considered. Factors in labeling of erythrocytes by 51Cr according to the original Signette-method of Kuni et al. resulting in an increase of free 51Cr in serum were studied. A 40% Sorbitol solution (250 ml) was infused in 20 min to healthy volunteers under standard conditions, the circulating blood volume was measured immediately prior to and 5 and 15 min after the infusion of the test solution. The circulating blood volume was determined by the dilution technique using 51Cr-labeled erythrocytes and the half-automatic equipment Volemetron (Ames-Atomium, Amsterdam). The erythrocytes were labeled with 51Cr using the original Signette-method of Kuni et al. Five min after the infusion the circulating blood volume was determined without injection of an additional 51Cr-dosis; 15 min after the infusion the blood-volume-determination was carried out by a new dosis of 51Cr-labeled erythrocytes. The use of erythrocytes which were labeled with 51Cr according to the original Signette-method of Kuni et al. without washing after labeling is not applicable for the determination of changes in circulating blood volume due to infusion of hyperosmotic solutions. It will result in high values as in vitro studies earlier stated. The results of the experiments to determine the changes of circulating blood volume 15 min after the infusion of 250 ml of a 40% Sorbitol solution confirmed earlier results, that at this time the circulating blood volume is lowered, especially by shrinkage of the circulating red cell volume due to hyperosmolarity in serum due to infused Sorbitol. To be able to receive true values on the changes in circulating blood volume during and after the infusion of osmotherapeutics, it was necessary to repeat the study using 51Cr-labeled erythrocytes washed after labeling.