Mouse spleen cell populations involved in the mitogenic response to protein A
Kanno, T.; Nariuchi, H.; Fukuyama, T.; Matsuhasi, T.
Japanese Journal of Experimental Medicine 50(1): 45-51
1980
ISSN/ISBN: 0021-5031 PMID: 6966701 Document Number: 163568
The cellular components involved in the mitogenic response of mouse spleen cells to protein A were examined. The mitogenic response was almost abrogated by the removal of macrophages. The abrogated response could be restored by the addition of peritoneal exudate macrophages from syngeneic mice. The reconstitution experiments were carried out for studying the populations of lymphocytes responding to protein A. In these experiments, spleen cells were passed through a Sephadex G-10 column and then they were separated into nylon-wool-adherent and nonadherent cells. The nylon-wool-nonadherent cells were used as T and complement. B and T cells apparently responded to protein A, and T-B cell cooperation could possibly play some role in the response.