Simultaneous registration of pyridine nucleotides and flavoproteins in contracting myocardium by the luminescence method
Sarapul'tsev, E.I.
Tsitologiia 22(10): 1241-1244
1980
ISSN/ISBN: 0041-3771 PMID: 7445088 Document Number: 161731
A luminescent divice has been elaborated for a simultaneous registration of the dynamics of NADH oxidation and of flavoprotein reduction in the beating heart muscle. The device is based on a microfluorimeter with alternating luminescence excitation in two-wave lenths--365 and 410 nm. The technique proposed makes it possible to minimize the reciprocal contribution of either carrier to the resulting measurement of their fluorescence intensity.